1. Selection of zebrafish - Zebrafish are chosen for their transparency during embryonic stages, rapid development, and genetic similarities to humans. Their scalability makes them ideal for high-throughput testing in biological research.

 

2. Compound preparation - Peptides, proteins, or other compounds are prepared under sterile conditions. These compounds are selected based on specific research objectives, such as studying muscle growth, aging, regeneration, or stress responses.

 

3. Application methods - Microinjection: Direct injection of compounds into zebrafish embryos or larvae. - Water immersion: Compounds are dissolved into the zebrafish's aquatic environment for gradual absorption. - Feeding: Compounds are introduced via specially prepared food.

 

4. Controlled environments - Zebrafish are housed in carefully monitored aquariums with controlled lighting, temperature, and water quality to ensure a stable environment for experiments.

 

5. Real-time monitoring - Observations are recorded using 4K HD cameras and advanced imaging techniques, such as fluorescence or confocal microscopy. This allows for detailed tracking of physiological or behavioral changes.

 

6. Data collection and analysis - Metrics such as growth rate, neural activity, regeneration speed, and reproductive changes are documented. - Statistical analysis ensures reproducibility and validates the results for broader scientific application.

 

7. Ethical considerations - Experiments are designed to minimize stress and adhere to ethical guidelines for zebrafish research, ensuring the well-being of the test organisms. This structured approach provides valuable insights into biological processes while leveraging zebrafish as an accessible and scalable model.